Mode
Bacteria sfGFP
Coordinates
It's a fluorescent bacteria artwork interface which remotely programs the Reconfigurable Automation Cart (RAC) system at Ginkgo Bioworks.
Nanoliter-scale droplets of fluorescent bacterial culture are dispensed onto an agar plate and photographed under UV light after incubation.
Nanoliter-scale droplets of fluorescent bacterial culture are dispensed onto an agar plate and photographed under UV light after incubation.
Fluorescent Bacteria Photograph
1. Publish your designed agar plate to the website gallery.
2. Approved designs are queued for automated production.
Retrieval from Storage
Travel on Magnemotion
Culture Plate into Echo 525
Agar Plate into Echo 525
3. After incubating overnight, the artwork is photographed under UV light and posted to the gallery tab.
Expression Strain: E. coli, BL21(DE3)
Antibiotic: Chloramphenicol at 12.5 µg/mL
Agar Plates: OmniTray or Petri Plate with LB Agar (Lennox)
Plasmid Design: Fluorescent protein genes under control of a constitutive promoter (BBa_J23106), using a strong RBS (BBa_B0034), double terminator (BBa_B0015), and 7x His tag.
Benchling example.
Sequence Optimization: Codon-optimized for E. coli and S. cerevisiae.
Photographs: Taken with an iPhone 17, 395 nm Cut-on Longpass Filter, and 312 nm UV lamp. Exposure, contrast, brightness, saturation, sharpness adjusted as necessary.